Akademik Veri Yönetim Sistemi
Biological Trace Element Research, cilt.139, sa.3, ss.347-355, 2011 (SCI-Expanded, Scopus)
We investigated the effect of 17β-estradiol (E2) alone and separately vitamin E treatment on trace element status of rats following an ovariectomic operation. Forty rats were equally divided into four groups: Group 1, control, non-ovariectomized rats; Group 2, (OVX) rats, ovariectomized under general anesthesia; Group 3, (OVX+E2) rats, the group received a 40 μg kg-1 subcutan dose of E2 per day after ovariectomy; and Group 4, (OVX+ E2+vitamin E) rats, received the same E2 treatment, but with an additional 100 mg kg-1 intraperitoneal dose of vitamin E per day after ovariectomy. At the end of the 30-day experiment, the rats were sacrificed and their blood was collected for the measurement of zinc, copper, iron, phosphorus, selenium, magnesium, calcium, manganese, and chromium; copper-zinc superoxide dismutase (SOD); manganese-superoxide dismutase (Mn-SOD); glutathione peroxidase (Se-GSH-Px); and catalase (CAT). The levels of zinc, copper, iron, phosphorus, selenium, calcium, chromium, and manganese and activities of SOD, Mn-SOD, Se-GSH-Px, and CAT were lower in the OVX than in the control group, but magnesium level was unaffected. However, zinc, copper, iron, phosphorus, selenium, calcium, chromium, and manganese levels and SOD, Mn-SOD, Se-GSH-Px, and CAT activities were higher under separate E2 and E2+vitamin E treatments. The level of magnesium in the treated-OVX groups was not different than in the OVX group. In conclusion, E2 treatment has an ameliorating effect on the trace element status in OVX, and this effect may be enhanced with the addition of vitamin E. © Springer Science+Business Media, LLC 2010.