Akademik Veri Yönetim Sistemi
Turk Hijyen ve Deneysel Biyoloji Dergisi, cilt.78, sa.3, ss.265-272, 2021 (Scopus, TRDizin)
Objective: Enterococci are one of the most commonly detected agents in community and hospital-acquired infections. Urinary tract infections are the most common form of enterococcal infections. Because enterococci are characterized by natural and acquired resistance to numerous antibiotics, there are serious problems in the treatment of infections caused by these bacteria. In our study, it was aimed to determine the resistance rates of enterococcal strains isolated from urine samples to various antibiotics within one year. Methods: 300 enterococci strains isolated from urine samples sent to Samsun Training and Research Hospital Microbiology Laboratory between January and December 2018 from various services and polyclinics were included in the study. The identification of bacteria and their antibiotic susceptibility were made by using the VITEK 2 system (BioMerieux, France) and conventional methods. Minimum inhibitory concentrations (MIC) of isolates with vancomycin resistance were determined by using the E-test (BioMerieux, France). Sensitivity results were determined based on European Committee on Antimicrobial Susceptibility Testing (EUCAST) criteria. Results: Of isolated 300 strains, 185 (61.6%) were identified as Enterococcus faecalis, 80 (26.6%) were Enterococcus faecium, 30 (10.0%) were Enterococcus spp., 4 (1.3%) were Enterococcus gallinarum and 1 (0.3%) was Enterococcus raffinosus. 174 (58%) of the 300 patients whose strains were isolated were female and 126 (42%) were male. Of the 238 isolates isolated from inpatients, 40% were isolated from the intensive care unit, 28% from surgical clinics, 14% from internal clinics, and 18% from the palliative clinic. For strains isolated from polyclinic patients, the rates are as follows: 13% from emergency clinics, 16% from internal diseases, and 67% from surgical polyclinics. Antibiotic resistance rates of E. faecalis and E. faecium is determined as 8.1% and 95% for ampicillin, 44.8% and 93.7% for ciprofloxacin, 0.5% and 18.7% for vancomycin, 0.5% and 18.7% for teicoplanin, 0% and 2.5% for linezolid, 0% and 1.25% for tigecycline, and 35.6% and 60% for high-level gentamycin respectively. Antibiotic resistance rates of 30 isolates defined as Enterococcus spp using conventional methods were determined as 33.8% for ampicillin, 73.3% for ciprofloxacin, 10% for vancomycin, 10% for teicoplanin, and 40% for high-level gentamicin. Conclusion: High resistance to ciprofloxacin, vancomycin and teicoplanin in enterococci isolated in our hospital limits the use of these antibiotics. Inappropriate use of antibiotics may increase vancomycin-resistant enterococcal colonization, leading to nosocomial infections.