Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, cilt.102, sa.2, ss.269-276, 2012 (SCI-Expanded, Scopus)
A novel actinobacterium, strain N9999 T, was isolated from soil and its taxonomic position determined using a polyphasic approach. The organism formed abundant aerial hyphae that differentiated into spherical spore vesicles. The cell wall contained mesodiaminopimelic acid; the whole-cell sugars were galactose, glucose, mannose, madurose and ribose; the predominant menaquinones MK-9 (H 2) and MK-9 (H 4); the major phospholipids phosphatidylethanolamine, diphosphatidylglycerol, a phosphaglycolipid and phosphatidylinositolmannosides; while the cellular fatty acids were rich in iso-C 14:0, C 15:0, cis-9-C 17:1, iso-C 16:0 and 10-methyl C 17:0 components. Phylogenetic analyses based on an almost complete 16S rRNA gene sequence indicated that strain N9999 T was closely related to a group that consisted of Streptosporangium pseudovulgare DSM 43181 T and Streptosporangium nondiastaticum DSM 43848 T. However, DNA-DNA relatedness and phenotypic data demonstrated that strainN9999 T was clearly distinguished fromall closely related Streptosporangium species. The combined genotypic and phenotypic data demonstrate conclusively that the isolate should be classified as a new species of Streptosporangium. © Springer Science+Business Media B.V. 2012.