Association of Matrix Metalloproteinase-3 (MMP-3) Gene Methylation Status with the Risk of Developing Achilles Tendonitis: A Preliminary Study Matriks Metalloproteinaz-3 (MMP-3) Geni Metilasyon Durumunun Aşil Tendinit Gelişmi Riski ile İlişkisi: Bir Ön Çalışma

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Tural E., Betul Celik Z. B., Tekcan E., Tural Ş.

Van Medical Journal, cilt.30, sa.1, ss.22-26, 2023 (Scopus, TRDizin) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 30 Sayı: 1
  • Basım Tarihi: 2023
  • Doi Numarası: 10.5505/vtd.2023.88864
  • Dergi Adı: Van Medical Journal
  • Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.22-26
  • Anahtar Kelimeler: Epigenomics, methylation, tendonopathy
  • Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
  • Samsun Üniversitesi Adresli: Evet

Özet

Introduction: In this study, we aimed to examine the relationship between the MMP-3 gene promoter methylation status and the risk of developing achilles tendinitis in endurance athletes. Materials and Methods: A total of 20 endurance athletes with achille tendonitis and 20 sedantary controls were involved in the study. DNA isolation and bisulfite modification were performed from peripheral blood samples of all volunteers. Using methylation-specific primers designed for MMP-3 gene promoters, methylation profiles were analyzed by the methylation specific polymerase chain reaction method. Results: In the MMP-3 gene promoter methylation analysis, the methylation frequency was found to be 75% in athletes with Achilles tendinitis, while it was found to be 100% in the control group. MMP-3 promoter region methylation status in the patient group was found to be hypomethylated compared to the control group. Discussion and Conclusion: The hypomethylated status of the MMP-3 gene promoter may have a role in developing achilles tendonitis in athletes. Furher and larges studies are needed to confirm our results.